TB-500 Reconstitution Calculator

A synthetic fragment of the naturally occurring thymosin beta-4 protein. Preclinical studies explore roles in cell migration, angiogenesis, and tissue repair.

Informational only. Not medical advice. Consult a licensed healthcare provider before starting, changing, or stopping any protocol.

Reconstitution calculator

TB-500

Syringe type
Your dose
Draw 100 units
That's 1 mL of your reconstituted solution · Concentration 2,500 mcg/mL
This vial gives you 2 doses at 2,500 mcg
0102030405060708090100100 units
Math is open source · Educational use only · Not medical advice

About TB-500

Not FDA-approved. Sold as a research chemical. Regulatory status varies by jurisdiction. Users are responsible for legal compliance.

TB-500 refers to a synthetic peptide fragment of thymosin beta-4, a 43-amino-acid protein found naturally in human cells. The TB-500 fragment corresponds to the active region of thymosin beta-4 and has been studied in preclinical research for its effects on cell migration, angiogenesis (blood vessel formation), wound healing, and cardiac tissue repair in animal models. Clinical data in humans remains limited.

Users tracking TB-500 in this app typically log 2,000–5,000 mcg per injection, twice weekly (e.g., Monday/Thursday). The 72-hour half-life is significantly longer than BPC-157's, which is why the dosing frequency is much less frequent — same or higher peak concentration achievable with fewer injections. TB-500 is not an FDA-approved drug; it is classified as a research chemical in most jurisdictions, with regulatory status varying by country and state.

Frequently asked questions

Why is TB-500 dosed less frequently than BPC-157?
TB-500 has a half-life of approximately 72 hours — much longer than BPC-157's 4 hours — which means the compound persists at useful concentrations for several days after injection. Twice-weekly dosing is the typical logged pattern.
What's a common vial + water volume?
5 mg vials reconstituted with 2 mL of bacteriostatic water yield 2,500 mcg/mL. A 2,500 mcg dose is 1 mL (100 units on a U-100 insulin syringe). Users often split across two injection sites for volume tolerability.
Is TB-500 the same as Thymosin Beta-4?
TB-500 is a synthetic fragment of Thymosin Beta-4 (TB4), corresponding to the active region. Full TB4 protein and TB-500 fragment have overlapping but not identical activity profiles. Most "TB-500" sold is the fragment form.

Sources

  1. Crockford D, et al. "Thymosin beta4: structure, function, and biological properties supporting current and future clinical applications." Ann N Y Acad Sci. 2010. PMID: 20955334
  2. Goldstein AL, et al. "Thymosin β4: a multi-functional regenerative peptide." Expert Opin Biol Ther. 2012. PMID: 22512638
  3. Sosne G, et al. "Thymosin beta 4 and the eye: the journey from bench to bedside." Expert Opin Biol Ther. 2015. PMID: 26174357

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