CJC-1295 / Ipamorelin
CJC-1295 / Ipamorelin Reconstitution and Dosing Guide
Blend vial vs. separate vial reconstitution math for CJC-1295/Ipamorelin, DAC vs. no-DAC explained, injection timing, doses per vial, and stack tracking.
Informational only. Not medical advice. Consult a licensed healthcare provider before starting, changing, or stopping any protocol.
The quick answer
CJC-1295/Ipamorelin blend vials (typically 5 mg + 5 mg = 10 mg total) reconstituted with 2 mL bac water: 200 mcg of each peptide per injection = 8 units on a U100 syringe. For separate vials, calculate each independently. Use the calculator for your specific setup.
Blend vials vs. separate vials
CJC-1295 and Ipamorelin are almost always used together — they have complementary mechanisms that produce a synergistic growth hormone (GH) pulse when co-administered. They come in two configurations:
- Pre-blended vial — a single vial containing both peptides at a fixed ratio (commonly 5 mg CJC-1295 + 5 mg Ipamorelin). You reconstitute once and draw both peptides in a single injection.
- Separate vials — each peptide in its own vial. You reconstitute each separately and either draw from both into one syringe before injecting, or inject at the same site consecutively.
The math for a blended vial treats the combined weight as the vial strength. For separate vials, each peptide follows its own calculation independently.
Blended vial calculation
For a 5 mg CJC-1295 + 5 mg Ipamorelin blended vial (10 mg total) + 2 mL bac water:
- Total concentration: 10,000 mcg ÷ 2 mL = 5,000 mcg/mL total(2,500 mcg/mL of each peptide, assuming 1:1 ratio).
- For 200 mcg of each (400 mcg total dose): 400 mcg ÷ 5,000 mcg/mL × 100 = 8 units.
- For 100 mcg of each (200 mcg total dose): 4 units.
- For 300 mcg of each (600 mcg total dose): 12 units.
Separate vial calculation
For a 5 mg CJC-1295 vial + 1 mL bac water, targeting 100 mcg CJC-1295:
- Concentration: 5,000 mcg ÷ 1 mL = 5,000 mcg/mL.
- Draw: 100 mcg ÷ 5,000 mcg/mL × 100 = 2 units.
For a 5 mg Ipamorelin vial + 1 mL bac water, targeting 200 mcg Ipamorelin:
- Concentration: 5,000 mcg ÷ 1 mL = 5,000 mcg/mL.
- Draw: 200 mcg ÷ 5,000 mcg/mL × 100 = 4 units.
Total draw into one syringe (or two consecutive injections): 2 + 4 = 6 units total for 100 mcg CJC + 200 mcg Ipa.
CJC-1295 with DAC vs. without DAC
CJC-1295 comes in two forms with very different half-lives:
- CJC-1295 without DAC (also called Mod GRF 1-29) — half-life of approximately 30 minutes. Used for once- or twice-daily pulsed injections that mimic the body's natural GH release pattern. This is the form most commonly paired with Ipamorelin.
- CJC-1295 with DAC (Drug Affinity Complex) — half-life of approximately 6–8 days. Used for once- or twice-weekly injections. Produces a sustained GH increase rather than a pulsed release. Usually not combined with Ipamorelin in standard protocols because the timing mismatch reduces the pulsing advantage.
If your vial says "CJC-1295" without specifying, it is most likely the no-DAC version used for daily dosing. Confirm with your pharmacy.
Dosing timing: why it matters more than the amount
CJC-1295 (no DAC) + Ipamorelin is most effective when injected in a fasted state — either first thing in the morning before eating, or at bedtime (last meal ≥2 hours prior). The reasoning: circulating insulin blunts GH secretion. Injecting when insulin is at its nadir maximizes the GH pulse the combination produces.
This is different from most peptide protocols where timing is less critical. It's also why tracking injection time matters as much as tracking injection amount.
How many doses per vial?
A 5 mg + 5 mg blended vial (10 mg total) reconstituted with 2 mL bac water at 200 mcg of each peptide per injection (400 mcg total, 8 units):
- 10,000 mcg total ÷ 400 mcg per injection = 25 doses.
- At once daily: 25-day supply.
- At twice daily: 12-day supply.
Storage
Lyophilized CJC-1295 and Ipamorelin powder: refrigerate at 2–8 °C or store at room temperature away from heat and light. Shelf life of several months when kept properly.
Reconstituted solution: refrigerate immediately, use within 28 days. Do not freeze. Do not expose to direct sunlight.
Running a full GH stack in My Pep Calc
Many people running CJC-1295/Ipamorelin also stack other compounds — BPC-157 for tissue repair, or a GLP-1 for body composition. Each compound has its own reconstitution setup, its own dosing schedule, and its own injection sites.
This is where protocol complexity scales geometrically. One compound is simple. Two is manageable. Three or four compounds with different vial concentrations, injection timing windows, and half-lives create real error surface.
My Pep Calc tracks each compound independently in the same dashboard — reconstitution math, dose log, site rotation, and half-life visualization — so nothing falls through the cracks as your stack grows.
Frequently asked questions
- How many units do I draw for 200 mcg each of CJC-1295 and Ipamorelin?
- For a standard 5 mg + 5 mg blended vial reconstituted with 2 mL bac water: 8 units on a U100 syringe (400 mcg total ÷ 5,000 mcg/mL × 100). If you used a different bac water volume, use the My Pep Calc calculator with your specific numbers.
- Should I use CJC-1295 with DAC or without DAC?
- For daily pulsed dosing combined with Ipamorelin, use CJC-1295 without DAC (Mod GRF 1-29), which has a 30-minute half-life and produces a natural-pattern GH pulse. CJC-1295 with DAC has a 6–8 day half-life and is used for weekly injections without Ipamorelin pairing in standard protocols. Confirm your prescription with your provider.
- When should I inject CJC-1295/Ipamorelin?
- Most protocols call for injection in a fasted state — either in the morning before eating or at bedtime at least 2 hours after the last meal. Circulating insulin suppresses GH secretion, so injecting when insulin is lowest maximizes the GH pulse.
- Can I combine CJC-1295/Ipamorelin with BPC-157 or tirzepatide?
- Yes. CJC-1295/Ipamorelin is a GH secretagogue stack focused on GH pulse optimization. BPC-157 is a tissue repair peptide; they operate through different mechanisms and can be run simultaneously. Tirzepatide (GLP-1/GIP) is a metabolic compound. Stacking multiple compounds requires careful dose tracking — use My Pep Calc to manage each independently.
- What is the typical CJC-1295/Ipamorelin protocol length?
- Common protocol lengths range from 3 to 6 months, with some practitioners cycling off for 4–8 weeks before resuming. Protocol design is your provider's call based on your goals and labs.
- How long does CJC-1295/Ipamorelin take to work?
- This is a question for your prescribing provider, not a calculator. We track protocols; we don't evaluate outcomes or make efficacy claims.
Sources
- Ionescu M, Frohman LA. Pulsatile secretion of growth hormone (GH) persists during continuous stimulation by CJC-1295, a long-acting GH-releasing hormone analog. J Clin Endocrinol Metab. 2006;91(12):4792-4797.
- Raun K, et al. Ipamorelin, the first selective growth hormone secretagogue. Eur J Endocrinol. 1998;139(5):552-561.
- Alba M, et al. Once-monthly administration of CJC-1295, a long-acting growth hormone-releasing hormone (GHRH) analogue, normalizes growth in the GHRH knockout mouse. Am J Physiol Endocrinol Metab. 2006;291(6):E1290-4.
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