BPC-157
BPC-157 Reconstitution: How Many Units to Draw
BPC-157 vial reconstitution guide: reference table of units for common setups, the formula, doses per vial, half-life, storage, and injection site tracking.
Informational only. Not medical advice. Consult a licensed healthcare provider before starting, changing, or stopping any protocol.
The quick answer
5 mg BPC-157 vial + 2 mL bac water + 250 mcg dose = 10 units on a U100 syringe. For 500 mcg: 20 units. For 1 mL bac water at 250 mcg: 5 units. Use the BPC-157 calculator for any other combination.
BPC-157 vial sizes and common setups
BPC-157 is sold in two standard vial sizes: 5 mg and 10 mg. Common reconstitution setups and the resulting draws for a 250 mcg dose:
| Vial size | Bac water added | Concentration | Units for 250 mcg | Units for 500 mcg |
|---|---|---|---|---|
| 5 mg | 1 mL | 5,000 mcg/mL | 5 units | 10 units |
| 5 mg | 2 mL | 2,500 mcg/mL | 10 units | 20 units |
| 5 mg | 3 mL | 1,667 mcg/mL | 15 units | 30 units |
| 10 mg | 2 mL | 5,000 mcg/mL | 5 units | 10 units |
| 10 mg | 4 mL | 2,500 mcg/mL | 10 units | 20 units |
The most common setup in the community is a 5 mg vial + 2 mL bac water. It keeps concentrations at an easy-to-measure level and the draw amounts land in a comfortable syringe range (10–20 units for typical doses).
The formula
This is the same formula used for all peptides — BPC-157 is not special here.
- Concentration (mcg/mL) = Vial strength (mcg) ÷ Bac water (mL)
5 mg vial = 5,000 mcg. 5,000 ÷ 2 mL = 2,500 mcg/mL. - Volume (mL) = Target dose (mcg) ÷ Concentration (mcg/mL)
250 mcg ÷ 2,500 mcg/mL = 0.10 mL. - Syringe units (U100) = Volume (mL) × 100
0.10 mL × 100 = 10 units.
How many doses per vial?
A 5 mg vial contains 5,000 mcg total. At 250 mcg/dose: 20 doses. At 500 mcg/dose: 10 doses. At 1,000 mcg/dose (1 mg): 5 doses.
A 10 mg vial at 250 mcg/dose yields 40 doses. Most researchers run once- or twice-daily injection schedules, so a 5 mg vial lasts 10–20 days depending on dose.
BPC-157 injection routes
BPC-157 is most commonly administered via subcutaneous (SubQ) injection, though some protocols use intramuscular (IM) injection, particularly for musculoskeletal applications. The reconstitution math is the same regardless of injection route.
For SubQ injection, common sites are the abdomen (2 inches from the navel), thigh, or upper arm. Rotating sites between injections prevents localized irritation. My Pep Calc's site rotation tracker maintains the rotation automatically.
Half-life and dosing frequency
BPC-157 has a short half-life, estimated at approximately 4 hours in published research. This is why protocols typically call for once or twice daily dosing rather than a weekly injection schedule.
What this means in practice: a morning injection produces peak circulating levels by mid-morning with near-complete clearance by evening. A second evening injection maintains coverage overnight. Whether your protocol calls for once or twice daily is a question for your prescribing provider.
See the half-life chart to visualize BPC-157's clearance curve alongside any other compound you're running.
Storage after reconstitution
Reconstituted BPC-157 should be refrigerated at 2–8 °C and used within 28 days. Lyophilized (unreconstituted) powder is more stable; store at or below room temperature away from light and moisture until ready to reconstitute.
Do not freeze reconstituted BPC-157. Do not expose to direct sunlight. If the solution becomes cloudy, precipitates, or changes color, discard it.
FDA and regulatory status
BPC-157 is not FDA-approved as a finished drug product. It is classified as a research compound. In the United States, it is available from licensed 503A/503B compounding pharmacies in some states, subject to prescriber order, and from research chemical suppliers. Regulatory status is actively evolving — check current guidance from your prescribing provider.
My Pep Calc is a tracking tool, not a pharmacy or provider. We do not sell peptides, endorse suppliers, or provide medical advice. All dosing decisions should be made with a licensed healthcare provider.
Tracking a BPC-157 protocol
A BPC-157 protocol typically runs 4–12 weeks. Tracking what matters:
- Dose per injection and timing — especially relevant for twice-daily protocols where morning and evening doses may differ.
- Injection site rotation — repeated injection at the same site causes local fibrosis over time.
- Vial inventory — knowing how many doses remain prevents running out mid-protocol.
- Protocol timeline — noting when the protocol started and milestones helps evaluate subjective outcomes over time.
My Pep Calc handles all four in a single dashboard. The reconstitution calculator feeds your dose log, the dose log feeds site rotation, and the protocol timeline pulls from actual logged events rather than a separate notes file.
Frequently asked questions
- How many units do I draw for 250 mcg of BPC-157?
- It depends on your bac water volume. With a 5 mg vial and 2 mL bac water: 10 units. With 1 mL bac water: 5 units. With 3 mL bac water: 15 units. The My Pep Calc BPC-157 calculator gives you the exact number for any combination of vial size, bac water, and dose.
- What size needle do I use to reconstitute BPC-157?
- Use an 18–23 gauge needle to draw up the bacteriostatic water (larger bore makes drawing easier) and a 27–31 gauge insulin needle for injection. Some people use the same syringe for reconstitution and injection if drawing from a small-volume vial.
- Can I mix BPC-157 with TB-500 in the same vial?
- Yes, BPC-157 and TB-500 (Thymosin Beta-4) are commonly run together and can be reconstituted in the same vial. The formula changes: each peptide's concentration is based on its own amount divided by the total shared bac water volume. My Pep Calc handles blended vials with per-compound dose calculation.
- How long does BPC-157 stay active after injection?
- BPC-157 has an estimated half-life of approximately 4 hours, meaning roughly half the injected amount is cleared every 4 hours. After about 20 hours, less than 5% remains active. This is why protocols typically call for daily or twice-daily dosing rather than weekly injections.
- Does BPC-157 need to be refrigerated?
- Lyophilized (unreconstituted) powder can be stored at room temperature away from heat and light. Once reconstituted, it must be refrigerated at 2–8°C (35–46°F) and used within 28 days. Do not freeze the reconstituted solution.
- What is the shelf life of a reconstituted BPC-157 vial?
- With bacteriostatic water containing 0.9% benzyl alcohol, reconstituted BPC-157 is generally considered stable for 28 days refrigerated. Some sources cite up to 56 days. When in doubt, use the more conservative estimate and track your reconstitution date in My Pep Calc.
Sources
- Sikiric P, et al. BPC 157: a review of central nervous system effects. Curr Neuropharmacol. 2014;12(6):502-515.
- Chang CH, et al. The promoting effect of pentadecapeptide BPC 157 on tendon healing involves tendon outgrowth, cell survival, and cell migration. J Appl Physiol. 2011;110(3):774-780.
- Pevec D, et al. Impact of pentadecapeptide BPC 157 on muscle healing impaired by systemic corticosteroid application. Med Sci Monit. 2010;16(3):BR81-88.
- United States Pharmacopeia. USP General Chapter <797>: Pharmaceutical Compounding — Sterile Preparations.
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